TutorialsΒΆ

This tutorial shows how to run a image-based profiling pipeline using Pycytominer. Using IPython notebooks, it walks through the following steps:

  1. Downloading a dataset of single cell CellProfiler profiles.

  2. Processing the profiles using PyCytominer. This includes the following steps:
    1. Data initialization

    2. Single cell aggregation to create well-level profiles

    3. Addition of experiment metadata to the well-level profiles

    4. Profile normalization

    5. Feature selection

    6. Forming consensus signatures

  3. Evaluating the profile quality using cytominer-eval.